Activated Carbon as Support for Lipase Immobilization

Mohd Basyaruddin Abdul Rahman, Mahiran Basri, Mohd Zobir Hussein, Raja Nor Zaliha Raja Abdul Rahman, Yau Kim Yan and Abu Bakar Salleh.

Centre for Research in Enzyme and Microbial Technology, Faculty of Science and Environmental Studies,

Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.


Lipase from Candida rugosa was immobilized onto four different types of activated carbon; KI/2030, KI/3040, KI/5060 and KI/6070. The immobilized lipase was used in the esterification of oleic acid and 1-butanol in hexane. The effects of difference pore sizes, surface area, reaction temperature, thermostability of the immobilized lipases, storage stability in organic solvent and leaching studies were investigated. Among the four samples, KI/6070 gave the highest activities and stability in all the parameters investigated. Immobilized lipases generally exhibit activities higher than the native lipase for the parameters studied, with optimum temperature of 40° C. Immobilized lipases are more stable than native lipase in hexane at room temperature up to 12 days. Leaching study proved that the immobilization of lipase using physical adsorption is cheap and easy. This method was found to be suitable for the attachment of enzyme on the support.